Measurement of Glutamate Uptake and Reversed Transport by Rat Synaptosome Transporters
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چکیده
منابع مشابه
Measurement of glutamate uptake and reversed transport by rat synaptosome transporters.
To establish an assay system for evaluation of the uptake and reversed transport of glutamate, we examined the effects of Na(+)-concentration and pharmacological agents on the extracellular glutamate concentration ([Glu](o)) in rat cortical synaptosomes in vitro. There was a decrease and increase of the [Glu](o) at high and low Na(+) concentrations, respectively, in a Ca(2+)-free medium. The ch...
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4. Binder, L. I . , Frankfurter, A. & Rebhun. L. I. ( 1985) J . Cell Biol. 101.1371-1378 5 . Wischik. C. M., Novak, M., Thogersen, H. C., Edwards, P. C., Runswick, M. J., Jakes, R., Walker, J. E., Milstein, C., Roth, M. & Klug, A. ( 1 988) /'roc. Natl. Acad. Sci. U.S.A. 85,4506-45 I 0 6. Bre. M. H. & Karsenti, E. (1990) C'eN Motil. C'ytoskel. in the press 7. Mitchison, T. & Kirschner, M. (1984)...
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The transport of glutamate across the plasma membrane is coupled to the movement of cations (Na+, K+, and H+) that are necessary for glutamate uptake and transporter cycling as well as anions that are uncoupled from the flux of glutamate. Although the relationship between these coupled (stoichiometric) and uncoupled (anion) transporter currents is poorly understood, transporter-associated anion...
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Within neurons, mitochondria are nonuniformly distributed and are retained at sites of high activity and metabolic demand. Glutamate transport and the concomitant activation of the Na(+)/K(+)-ATPase represent a substantial energetic demand on astrocytes. We hypothesized that mitochondrial mobility within astrocytic processes might be regulated by neuronal activity and glutamate transport. We im...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2004
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.27.813